Salting-out of enzymes with ammonium sulphate
نویسندگان
چکیده
منابع مشابه
salting-out procedure
DNA from archival Papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human P-globin, human P-actin and human papillomavirus LI genes. Only negligible differences in amplification efficiency were observed b...
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Although the understanding of ion specific effects on the aqueous solubilities of biomolecules is crucial for the development of many areas of biochemistry and life sciences, a consensual and well-supported molecular picture of the phenomena has not yet been established. Mostly, the influence of cations and the nature of the molecular interactions responsible for the reversal of the Hofmeister ...
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متن کامل1H NMR and molecular dynamics evidence for an unexpected interaction on the origin of salting-in/salting-out phenomena.
By employing (1)H NMR spectroscopy and molecular simulations, we provide an explanation for recent observations that the aqueous solubilities of ionic liquids exhibit salting-out to salting-in regimes upon addition of distinct inorganic salt ions. Using a typical ionic liquid [1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide], we observed the existence of preferential specific inte...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 1971
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.260130513